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Plantstress.com

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Casting a critical eye on plant stress research

For the benefit of learning

By Abraham Blum

 

"You must learn from the mistakes of others. You can't possibly live long enough to make them all yourself."  Sam Levenson (1911-1980)    

 

INTERPRETATIONS

o    Basic problem in the interpretation of plant water status in plants under stress – an example, 1995 (see letters)

o          ... and a letter to Plant Physiology –1996

o          ... and a letter to Plant Physiology – 2002

o    Flawed evaluation and interpretation of transgenic plants for drought resistance – an example

o    Plant sterility under drought stress is caused by antioxidant inefficiency?

o    Drought resistant varieties as an experimental tool to prove a physiological hypothesis – a frequent pitfall

o    Need to understand cause and effect in analyzing drought resistance

o    The greenhouse as a possible source of experimental error

o    Does ABA Sensitivity Enhance Drought Resistance? - The Dilemma

           + Overexpression of ABA regulated genes cause photosynthesis, growth and yield reduction in potato.

o   The disturbing pot size effect in evaluating plant response to drought stress. (pots with water deficit raises the need to carefully consider the results)

o    Gene expression and plant water status, revisited

 

DEFECTIVE PROTOCOLS IN STRESS RESEARCH

Note: In many cases this website called the attention of journal editors to the defective/absurd methodology in the accepted published papers. It was pointed out to editors that there is evidence that published flawed protocols are accepted at face value as legitimate methods especially by young scientists. Editors never responded.

   

O     Improper measurements of plant water status are still being used (in Physiologia Plantarum)

O     Improper measurements of plant water status are still being used (#2 ) (in Euphytica)

O     Irrelevant stress protocols used and reported in genomic research (in Plant Molecular Biology)

O     Irrelevant stress protocols used and reported in genomic research-II (in Planta)

O     Physiological and developmental problems encountered by in vitro cultured plants (in Plant Growth Regulation)

O     In a genomics study drought stress was applied to leaf discs dried in air for 2 hours (in Theoretical and Applied Genetics)

O     And still unrealistic drought stress treatment in a genomics study – seedlings dried for 0.5 to 2h (in Theoretical and Applied Genetics)

O     Irrelevant drought stress treatments and interpretations in plant physiology experiments – still in view (in Plant Growth Regulation)

O     Gene expression under drought stress is assessed by pulling seedlings out of nutrient solution and desiccating them in air. (in Journal of Experimental Botany)

O     A new inventive “drought stress” protocol  (in Molecular Breeding)

 

CONTRARIAN DATA AND VIEWS

O    What proportion of declared QTL in plants are false?

O    “Why Genetic Engineering Is Not Solving Agriculture's Drought Problem in a Thirsty World” – a critique

O    “Salt stress or salt shock: which genes are we studying?” – Issues with salinity gene-expression research.

O    Is Arabidopsis thaliana the proper model plant for research?

O    Small heat shock protein (HSP), a consensus factor of stress resistance, found to increase plant sensitivity to heat and salinity stress

O    Overexpression of TaNAC2D Displays Opposite Responses to Abiotic Stresses between Seedling and Mature Stage of Transgenic Arabidopsis

O    ROS are good. A view (by Ron Mittler, 2017).

O    Regulating Tradeoffs to Improve Rice Production – Certain genes may have both positive and negative effects on crop performance

O    The Involvement of Heat Shock Proteins in the Establishment of Tomato Yellow Leaf Curl Virus Infection.

 

 

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