AN ELECTRONIC POTOMETER FOR STUDYING PLANT WATER USE IN REAL TIME
Refael Aharon, Gad Galili, Abraham Blum12 and Yoram Kapulnik13
Department of Plant Science, the Weizmann Institute of Science, Rehovot 76100, Israel;
and Natural Resources Dept., ARO, the
2presently at www.plantstress.com
3Corresponding author email: email@example.com
In order to achieve accurate real-time monitoring of plant water use by means of a simple, multi-channel apparatus, we developed a Micro-Electronic Potometer (MEP). The instrument is built from six units each comprising two parallel vessels joined by a tube; one vessel accommodates a hydroponically grown plant; the other contains a float connected to a high-accuracy linear variable differential transducer (LVDT). Very small changes in water level are recorded by the LVDT output. The potometer responds to a change in water use as little as 1-mL. The instrument was tested by monitoring the water uptake rates of 3-week-old tobacco seedlings and detached tobacco plant organs, following exposure to changes in light intensity and addition of NaCl, HgCl2 or abscisic acid to the root medium. All of these treatments elicited the expected responses in water use within 3-20 min. The advantage of the instrument was also demonstrated in distinguishing between water use of several plant species, including Arabidopsis thaliana cv. Colombia C24 and young pine trees, under normal and stress conditions. This potometer enables the immediate, real-time evaluation of continuous changes in plant water use in response to modifications in the environments of the roots or shoots. Thus, it is a useful, low-cost laboratory instrument for diverse physiological research into plant-water relations.
Plant water status is a major plant stress that directly impacts on photosynthesis and growth rate. The mechanisms that regulate plant water use (PWU; defined as the total amount of water that a plant uses for growth and transpiration) form an important area of research into plant stress physiology and plant breeding.
Several methods have been reported for measuring water use and flow rates in plants. Water flux through the plant can be estimated by measuring changes in pressure potential at desired time intervals, by means of pressure probes (Makoto and Melvin, 2000; Steudle, 1993). Water use can also be evaluated by tracking the sap flow in the stem, which can be determined by the heat flux (Baker and van Bavel, 1987) or the heat pulse technique (Cohen et al., 1981). However, these methods are limited to plants or trees with rigid stems, so that most annual herbage plants cannot be measured easily, if at all. PWU is often evaluated by monitoring the weight loss of intact plants grown in pots, but this procedure is very inaccurate because of the relatively high weight of the pots and soil.
The potometer embodies an old method whereby whole plant roots or detached leaf petioles are immersed in water and the reduction in water level is taken to represent the water used (Pfeffer, 1881; Kaldenhoff et al., 1998). Some of the original potometers had very low resolution (So et al., 1976) and were able to use detached plant organs only, and had a short range of linearity or had to be used over an extended period of time (Lervine et al., 1996; Zwieniecki and Boersma, 1997). Also, these original potometers could not hold plants with their entire root systems (Rosene, 1937; Zwieniecki and Boersma, 1997), and did not allow the transient real-time detection of water use by the roots or shoots of the tested plants (Rosene, 1937; So et al., 1976). Thus, many of the available potometers were unsuitable for recording rapid small changes in real time PWU, such as those that occur in small Arabidopsis thaliana plants.
In this paper we describe a new research Micro-Electro Potometer (MEP), which appears to be sufficiently sensitive to measure small changes in PWU continuously, over short intervals and in real time, and which can be designed to accommodate plants of various sizes. This instrument is also relatively simple to construct and has the potential for use in high-throughput screening studies under diverse growth media conditions.
MATERIALS AND METHODS
tabacum L. cv.
Micro-electronic potometer technical description
The gauge was constructed of two glass
vessels joined by a 1-cm-diameter glass tube. One vessel – 24 mm in diameter –
was designed to accommodate a hydroponically grown
plant (the replaceable tube); the other – 30 mm in diameter – contains a
plastic float (Dalrin type – constant tube). The
float was connected to a high-accuracy linear variable differential transducer
(LVDT; type DG 2.5, from RS Components,
Figure 1.A. The micro-electronic potometer system: A. Overview.
Figure 1.B. The micro-electronic potometer system: B. Design. The main components of the instrument are the LVDT (linear variable differential transducer), the float, the changeable plant port (normal capacity, 190 mL), the plant support columns and the data logger. Detailed plans are available by writing to the corresponding author at firstname.lastname@example.org
Hydroponically grown plants were
used in all experiments. Three-week-old plants were transferred from the
greenhouse to hydroponic culture in a growth chamber
(Lumintron, from CE Ltd,
To evaluate the effect of salinity on PWU, the plants in the MEP tubes were exposed to tap water (control), or to 45 or 100 mM NaCl solution. In order to evaluate the effect of abscisic acid (ABA) on PWU, plants were monitored under optimal conditions (control treatment); the MEP tubes were then filled with 0.02 mM ABA solution and the water uptake of the plants was measured up to 18 h after treatment. The effect of mercury on PWU was evaluated by comparing the performance of plants under normal conditions (control treatment) with that of plants that had been incubated with 0.05 mM HgCl2 solution for 15 min before and during the measurement. The effect of darkness on PWU was tested by covering the plants with a sealed aluminum box or by switching off the lights in a lightproof room.
RESULTS AND DISCUSSION
Short- and long-term measurements
Figure 2.A. Calibration of the micro-electronic potometer. Accurately measured amounts of water were removed from the plant port (500 mL each time) and the MEP readings were recorded.
Figure 2. B. Sensitivity test. Successive aliquots of water were removed (1 mL each time) from the plant port, and the MEP readings were recorded (mL).
The system was full-scale calibrated at 240C, by recording LVDT output (in millivolts) against incremental water withdrawals from the plant port, and a linear curve was obtained (Figure 2A). To evaluate its sensitivity 44 aliquots of 1 mL of water which were successively removed from the plant port were compared with the respective LVDT output as mL water (Figure 2B). This association was very strong (R2=0.98) indicating high sensitivity of LVDT to water level changes. Figure 3 presents the comparative rates of water loss from the plant port as a result of evaporation (no plant in the plant port) and the presence of an Arabidopsis plant. In both cases, water loss from the port was linear with time up to the end of the test (15 h in this experiment). As expected water loss by plant transpiration was higher than evaporation. In all subsequent measurements, the evaporative water loss was subtracted from the measured water losses from ports containing plants. Small Arabidopsis plants with four full leaves were introduced into the plant port and the variation of water use over time was monitored for longer periods of time. The rate of water loss from the plant port was linear from the 3rd minute after initial detection of PWU to at least 96 h (data not shown). Therefore, long-term (days) as well as short-term (minutes) experiments can be accommodated by this MEP system. Furthermore, the relatively inexpensive and easily constructed and handled configuration allowed the use of several stations in parallel, thus facilitating the online analysis of several parallel treatments in one run.
Figure 3. Water use of a small Arabidopsis plant over 15 h as compared with the evaporation rate.
Figure 4. Regression of tobacco plant water use on leaf area.
Effect of plant attributes on water use
Regressions of water use on leaf fresh weight (F.W.), shoot F.W., total F.W. (root + shoot), and leaf area per plant (Figure 4) were linear and very significant with R2 values of 0.83, 0.82 and 0.78, respectively. These results are consistent with the normalizing units used in similar studies elsewhere (Herbst et al., 1996; Lambers et al., 1990; Poorter and Farquhar, 1994).
When the root volume of an individual tobacco plant was reduced (by successive trimmings) a positive association (R2 = 0.63) was found between the remaining root volume and plant water use (Figure 5).
The effect of light on the PWU of tobacco plants was studied by shifting the plants from light to total darkness. The air temperature at the canopy level was reduced by 2oC after 60 min in darkness. As shown in Figure 6, reduction in the PWU was evident after 50 min in darkness, as indicated by the change in the slope of the curve, compared with that of the control illuminated plant. PWU was reduced by nearly 87% in darkness, compared with that under illumination (data not shown). Our results suggest that closure of stomata occurred after 50 min in darkness, which is consistent with the previous results of Eun and Lee (1997).
Figure 5. The effect of root volume on the rate of plant water use. Roots of hydroponically grown tobacco plants were trimmed to differing extents and their water use measured.
Figure 6. The effect of darkness on water use of tobacco plants. Water use as a function of time by tobacco plants in light (control) or in darkness (Dark). Darkness was imposed after 175 min (double-headed arrow).
Effect of root-medium composition on water use
The MEP was used to test the effects of the addition of various chemicals to the root medium on the PWU. In testing the effects of salt stress in media containing 45 or 100 mM NaCl, PWU measurements began immediately after the beginning of the exposure to salt and continued for 60 min. The effect of NaCl on water use was already apparent within 3 min, and a slightly biphasic response was obtained in the salt-treated plants (Figure 7). Exposure of plant roots to 45 and 100 mM NaCl decreased steady state rate of water use by 24 and 50%, respectively, relative to non-stressed control plants (data not shown). Under mild salt stress (up to 60 mM NaCl) plants partially close their stomata (Maccrree et al., 1984) and reduce their root hydraulic conductivity (Rodrigues et al., 1997). Our results demonstrated the very fast response of the plant to salinity, and the ability of the MEP to track such fast and small responses in plant water use.
Figure 7. Effects of NaCl levels on water use by tobacco seedlings.
Figure 8. Effect of mercury on water use by tobacco plants: Tobacco seedlings were divided into two groups, one was pretreated with 50 mM HgCl2 (HgCl2) and the second with water (control) for 15 min. Both groups were removed into tap water and their water use rates were recorded as functions of time. Bars represent standard error of the means
Mercury is an inhibitor of certain classes of aquaporins (Maurel, 1997). A 15 min pre-treatment of the plants with 50 mM mercury as HgCl2, after which they were returned to tap water, resulted in a 71% reduction in the PWU rate (Figure 8). When plant water use was measured immediately after mercury application (i.e., the medium contained 50 mM mercury), a shift in water use was already observed after 3 min and the PWU continued to increase linearly with respect to time (data not shown). Thus, the MEP was effective in detecting reductions the symplastic water flux as caused by an aquaporin inhibitor.
When the root system was challenged with ABA,
two phases of response were detected: in the first 60 min there was a rapid
decrease in water use (by 42%) compared with that of control plants (Figure 9 -
inset); in the next 21 h there was a slower rate of decline in water uptake, to
a final reduction of 75%. The immediate response of plants to
The capability of the MEP to measure water uptake by detached organs was also tested (Table 1). In all cases, water use increased linearly with time, but at distinct rates for the different organs. This finding extends the options available in research into plant-water relations. For example, water use of detached roots is an indicator of the active pressure in the roots (Frensch and Steudle, 1989; Schobert and Komor, 1990; Steudle, 2000b) and it can also be used to investigate total root hydraulic conductance.
Table 1. Rates of water use of different plant species. Plants were grown hydroponically for 2 weeks and their leaf area measured. Means with different letters are significantly different at p<0.05.
9. Effect of
Water use by different plant species
The ability of the system to measure
water use by different plant species was tested. Arabidopsis (Arabidopsis
thaliana), tobacco (Nicotiana tabacum), wheat (Triticum
aestivum), corn (Zea
mays), tomato (Lycopersicon
esculentum), zucchini squash (Cucurbita
pepo) and bean (Vigna
radiata) were germinated and grown in pots for 10
days. Pine (Pimus pinea)
was germinated on seed germination paper (Anchor Paper Co.,
The results with arabidopsis also demonstrate the sensitivity of the instrument in detecting very small changes in plant water use. Moreover, the versatility of the instrument and the ability to replace a plant port with one of a different size may facilitate measurements with various plant species that differ in size and architecture.
Taken as a whole, our results demonstrate that this potometer system is suitable for the reliable, real-time measurement of small changes in plant water use over short (minutes) or long (hours) time intervals. In the light of these capabilities, this instrument can be seen to be a useful addition to the tools available for research into plant-water relations.
GG is the incumbent of the Bronfman Chair
of Plant Sciences at the Weizmann Institute. This
work was partially supported by grant No. 891-0155-99 from the Chief Scientist
of the Ministry of Agriculture and Rural Development,
Detailed plans for constructing this instrument are available by writing to the corresponding author at email@example.com
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