Notice: None of the methods listed here are under special recommendation by Plantstress.com
o Hormone Analysis – problems and solutions
§ Relations to Stress
§ PhotosynQ – a light portable leaf measurement system
§ An example of application (crop plant).
§ An example of application (Arabidopsis).
§ Check this site for instruments.
§ NDVI measurement – a practical example from CIMMYT
§ “FLIR-one” – A thermal imaging attachment for your smart phone (not yet verified for plant work)
o Plant Image Analysis – a collection of software
o Wheat Physiological Breeding II: A Field Guide to Wheat Phenotyping. (CIMMYT publication) (Large pdf file).
o Various Plant Biology Protocols (links on this site)
o New handbook for standardized measurement of plant functional traits worldwide (via DiverSus – mainly ecology)
Using pots in plant research – not so simple
o The pot experiment – How to avoid common pitfalls.
o Managing pot water status – a critique
o Applying experimental ‘drought’ to pots - the interpretation of results
o PLANT WATER STATUS
§ The pressure-volume curve – an important tool, not for large scale phenotyping
§ Detailed discussion of measurements and their theoretical basis – including more intricate methods such as thermocouple psychrometry
§ See remote sensing methods of plant stress - above
o A NEW NON-EVASIVE TURGOR RELATED MONITORING OF PLANT WATER STATUS
o Stomatal conductance (an example from CIMMYT; instruments may vary)
o Rapid colorimetric assay for epicuticular wax – Tested against the classical gravimetric method
o XYLEM VULNERABILITY TO CAVITATION AND EMBOLISM IN WOODY PLANTS
§ Water Soluble Carbohydrates (WSC) determination in stems (wheat example from CIMMYT).
o THE MANAGED DROUGHT STRESS ENVIRONMENT IN THE FIELD
o The Drought (Stress) Resistance Index – Estimating drought resistance in terms of yield or biomass
o Salt tolerance protocols; by Bado S. et al. 2016 (open access by IAEA and Springer)
o Chlorophyll content reduction under heat stress – see Leaf chlorophyll
o Chlorophyll Fluorescence (see under General Stress above)
o Pollen dysfunction under heat stress (see under General Stress above)
o Chlorophyll Fluorescence for chilling stress (see under General Stress above)
o Pollen dysfunction under chilling stress (see under General Stress above)
MINERAL TOXICITY STRESS
o Aluminum resistance screening assay (alfalfa)
o Aluminum resistance screening assay (pigeon pea)
o Aluminum resistance screening assay (cereals - review)
MINERAL DEFICIENCY STRESS
o Fingerprinting Antioxidative Activities in Plants (Contributed by Livia Saleh and Christoph Plieth)
o Oxidative stress and antioxidative systems