Selected methods in applied plant stress research
Notice: None of the methods listed here are under special recommendation by Plantstress.com
GENERAL STRESS METHODS
New handbook for standardized measurement of plant functional traits worldwide (via DiverSus – mainly ecology)
Soluble carbohydrates (SEE also WSC under drought)
Hormone Analysis – problems and solutions
- Relations to Stress
- imaging fluorescence
- integration with thermography.
- PhotosynQ – a light portable leaf measurement system
- An example of application (crop plant).
- An example of application (Arabidopsis).
- Check this site for instruments.
Proline analysis – The still popular fast method by Bates et al. (1973) – and more
SPECTRAL CANOPY SIGNATURES AND REMOTE SENSING FOR ASSESSING CROP STRESS AND PRODUCTIVITY:
- Leaf canopy temperature and its measurement with the infrared thermometer in the field
- Relations to water indices in soil and plant
- Relations to yield
- Equipment examples: GreenSeeker ; Yara-N Sensor
- NDVI measurement – a practical example from CIMMYT
- Spectral reflectance for phenotyping “stay-green” (“non senescence”).
- Thermal imaging (heat sensing camera) for identifying plant response to drought stress
- Thermal and spectral imaging of plant stress
- “FLIR-one” – A thermal imaging attachment for your smart phone (not yet verified for plant work)
Plant Image Analysis – a collection of software
Wheat Physiological Breeding II: A Field Guide to Wheat Phenotyping. (CIMMYT publication) (Large pdf file).
Various Plant Biology Protocols (links on this site)
To appreciate modern advanced instrumentation – See how leaf gas exchange was measured in the field in 1970’s
Using pots in plant research – not so simple
- The pot experiment – How to avoid common pitfalls.
- The Art of Growing Plants for Experimental Purpose
- Managing pot water status – a critique
- Applying experimental ‘drought’ to pots – the interpretation of results
PLANT WATER STATUS
- The pressure chamber for plant water status measurement
- Relative water content (RWC) as a measure of plant water status
- Detailed discussion of measurements and their theoretical basis – including more intricate methods such as thermocouple psychrometry
- Osmotic adjustment and its measurement
- Fast indirect estimate of osmotic adjustment for large breeding populations
- See remote sensing methods of plant stress – above
A NEW NON-EVASIVE TURGOR RELATED MONITORING OF PLANT WATER STATUS
Stomatal condcutance (an example from CIMMYT; instruments may vary)
Rapid colorimetric assay for epicuticular wax – Tested against the classical gravimetric method
o XYLEM VULNERABILITY TO CAVITATION AND EMBOLISM IN WOODY PLANTS
§ Conduits in wood – their measurements and the assessment of hydraulic vulnerability
§ Water Soluble Carbohydrates (WSC) determination in stems (wheat example from CIMMYT).
o THE MANAGED DROUGHT STRESS ENVIRONMENT IN THE FIELD
§ Rainout shelters – some basic principles of design and operation
o Salt tolerance protocols; by Bado S. et al. 2016 (open access by IAEA and Springer)
o Chlorophyll content reduction under heat stress – see Leaf chlorophyll
o Chlorophyll Fluorescence (see under General Stress above)
o Pollen dysfunction under heat stress (see under General Stress above)
MINERAL TOXICITY STRESS
o Aluminum resistance screening assay (pigeon pea)
o Aluminum resistance screening assay (cereals – review)
o Fingerprinting Antioxidative Activities in Plants (Contributed by Livia Saleh and Christoph Plieth)